Description

HL-60 cell
The HL-60 cell line was derived from a promyeloblast. The line was established from cells taken from a 36 years old female patient suffering of an acute promyelocytic leukemia.

HL-60 cells Production
HL-60 cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5×10⁶ cells/ml (cell viability: 93%-99%) under GLP conditions in our facility in Mons, Belgium. Cells are harvested in exponential phase.

Cell pellets are prepared by low speed centrifugation, rinsed with phosphate buffer saline, snap frozen in liquid nitrogen and stored at -85°C.

The cell pellets are not prepared in aseptic conditions and are not intended to be used as seed for a new culture.

Research Use Only
Our HL-60 cell pellets are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin. For published papers reporting the use of our extract, please check out our references list

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Sera used in the culture medium are CE certified from South American origin.