IpraCell has developed and reduced to practice a range of innovative and enabling technologies. This way, IpraCell is able to offer you high quality biologicals at the lowest cost.


Thanks to the IpraCell's innovative development and manufacturing process, all your results are reproducible between batches.


Our products are used worldwide by research or production entities for the study of biochemical processing, high throughput screening or purification of biological material from human origin.



Our HeLa cells are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin.


Selected papers using IpraCell HeLa and other mammalian cells


• Barabino, S.M.L., Sproat, B.S. and Lamond, A.I. (1992). Antisense probes tardeted to an internal domain in U2 snRNP specifically inhibit the second step of pre-mRNA splicing. Nucl. Ac. Res. 20, 4457-4464.

• Calvio, C., Neubauer, G., Mann, M. and Lamond, A.I. (1995). Identification of hnRNP P2as TLS/FUS using electrospray mass spectometry. RNA 1, 724-733.

• Damier, L., Domenjoud, L. and Branlant, C. (1997). The D1-A2 and D2-A2 pairs of splice sites from human immunodeficiency virus Type 1 are highly efficient in vitro, in spite of an unusual branch site. Biochem. Biophys. Res. Comm. 237, 182-187.

• Fabrizio, P., Laggerbauer, B., Lauber, J., Lane, W.S. and Lührmann, R. (1997). An evolutionary conserved U5 SnRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2. EMBO Journal 16, 4092-4106.

• Hackel, W.., Fischer, V. and Lührmann, R. (1994). A 69 kD protein that associates reversibly with the Sm core domain of several spliceosomal SnRNP species. J. Cell Biol. 124, 261-272.

• Kleren, M., Blank, V., Logest, F., Vandekerckhove, J., Lottspeich, F., Le Ball, O., Urban, M.B., Kourilsky, Ph., Bauerle, P.A. and Israel, A; (1990). The DNA binding subunit of NF-KB is identical to factor KBF, and homologous to the rel oncogene product. Cell 62, 1007-1018.

• Kreivi, J.P., Trinkle-Mulcahy, L., Lyon, C.E., Morrice, N.A., Cohen, P., Lamond, A.I. (1997). Purification and characterisation of p99, a nuclear modulator of protein phosphatase 1 activity FEBS Letters 420, 57­62.

• Laggerbauer, B., Lauber, J. and Lührmann, R. (1996). Identification of an RNA-dependent ATPase activity in mammalian U5 SnRNPs. Nucl. Ac. Res. 24, 868-875.

• Lamm, G.M., Blencowe, B.J., Sproat, B.S., Iribarren, A.M., Ryder, U. and Lamond, A.I. (1991). Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts. Nucl. Ac. Res. 19, 3193-3198.

• Lauber, J., Fabrizio, P., Teigelkamp, S., Lane, W.S., Hartmann, E. and Lührmann, R. (1996). The HeLa 200 kDa U5 SnRNP-specific protein and its homologue in Saccharomyces cerevisiae are members of the DEXH-box protein family of putative RNA helicases. EMBO Journal 15, 4001-4015.

• Lauber, J., Plessel, G., Prehn, S., Will, C.L., Fabrizio, P., Grôning, K., Lane, W.S. and Lührmann, R. (1997). The human U4/U6 SnRNP contains 60 and 90 kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p. RNA. 3, 926-941.

• Mermoud, J.E., Cohen, P. and Lamond, A.I. (1992). Ser/Thr-specific protein phosphatases are required for both catalytic steps of pre-mRNA splicing. Nucl. Ac. Res. 20, 5263-5269.

• Plessel, G., Fischer, U. and Lührmann, R, (1994). m3G cap hypermethylation of U1 small nuclear ribonucleoprotein (SnRNP) in vitro : evidence that the U1 small nuclear RNA-(guanosine-N2)­methyltransferase is a non-SnRNP cytoplasmic protein that requires a binding site on the Sm core domain. Molecular and Cellular Biology 14, 4160-4172.

• Rossmanith, W., Tullo, A., Potuschak, T., Karwan, R. (1995). Human mitochondrial tRNA processing. J. Biol. Chem. 270, 12885-12891.

• Ryder, U., Sproat, B.S. and Lamond, A.I. (1990). Sequence-specific affinity selection of mammalian splicing complexes. Nucl. Ac. Res. 18, 7373-7379.

• Salles, B., Provot, C., Calsou, P., Hennebelle, J., Gosset, I. and Fournié, G.J. (1995). A chemiluminescent microplate assay to detect DNA damage induced by genotoxic treatments. Analytical Biochemistry 232, 37-42.

• Ségault, V., Will, C.L., Sproat, B.S. and Lührmann, R. (1995). In vitro reconstitution of mammalian U2 and U5 snRNPs active in splicing : Sm proteins are essential for the formation of functional U2 and U5 SnRNPs. EMBO Journal 14, 7010-4021.

• Sirand-Pugnet, P., Durosay, P., Clouet d'Orval, B., Brody, E., Marie, J. (1995). R-Tropomyosin pre-mRNA folding around a muscle-specific exon interferes with several steps of spliceosome assembly. J. Mol. Biol. 251, 591-602.

• Will, C.L., Rümpler, S., Gunnewiek, J.K., Van Venrooij, W. and Lührmann, R. (1996). In vitro reconstitution of mammalian U1 SnRNPs active in splicing : the U1:C protein enhances the formation of early (E) spliceosomal complexes. Nucl. Ac. Res. 24, 4614-4623.

• Brehm, A., Miska, E.A., MacCance, D.J., Reid, J.L., Bannister, A.J., Kouzarides, T. (1998). Retinoblastoma protein recruits histone deacetylase to repress transcription. Nature 391, 591-601.

• Blencowe, B.J. and Barabino, S.M.L. (1995) Antisense Affinity depletion of RNP particles : In : Tymms, M.J. ed, Methods in Molecular Biology vol 37 : In vitro Transcription and Translation Protocols (pp 67-76). Humana Press inc publ (Totowa, NJ).

• Blencowe, B.J. and Lamond, A.I; (1999). Purification and depletion of RNP particles by antisense affinity chromatography. Meth. in Molecular Biology 118.

• Cairns, C.A. and White, R.J. (1998) p53 is a general represor of RNA polymerase III transcription. EMBO Journal 17, 3112-3123.

• Gell, D. and Jackson, S. P. (1999). Mapping of protein-protein interactions within the DNA-dependent protein kinase complex. Nucl. Ac. Res. 27, 3494-3502.

• Izzard, R.A., Jackson, S.P. and Smith, G.C.M. (1999). Competitive and non-competitive inhibition of the DNA-dependent protein kinase. Cancer Res. 59, 2581-2586.

• Lakin, N.D., Hann, B.C. and Jackson, S.P. (1999) The ataxia-telangiectasia related protein ATR mediates DNA-dependent phosphorylation of P53. Oncogene 68, 3989-3995.

• Smith, G.C., Cary, R.B., Lakin, N.D., Hann, B.C., Teo, S._H., Chen, D.J. and Jackson, S.P. (1999) Purification and DNA binding properties of the ataxia-telangiectasia gene product ATM. Proc. Natl. Acad. Sci. USA 96, 11134-11139.

• Smith, G.C., d'Adda di Fagagna, F., Lakin, N.D. and Jackson, S.P. (1999) Cleavage and inactivation of ATM during apoptosis. Molec. Cell. Biol. 19, 6076-6084.

• Hughes, P., Tratner, I., Ducoux, M., Piard, K., Baldacci, G. (1999) Isolation and identification of the third subunit of mammalian DNA polymerase d by PCNA-affinity chromatography of Mouse FM3A cell extracts. Nucleic Acids Res. 27, 2108-2114.

• Chew, S.L., Baginsky, L., Eperon, I.C. (2000) An exonic splicing silencer in the testes-specific DNA ligase III B exon. Nucleic Acid Res. 28, 402-410.

• Pethe, K., Aumercier, M., Fort, E., Gatot, C. and Menozzi, F.D. (2000) Characterization of the heparin binding site of the mycobacterial Heparin-binding hemagglutinin adhesin. J.Biol.Chem 275,1.

• Deplus, R., Brenner, C., Burgers, W.A., Putmans, P., Kouzarides, T., de Launoit, Y., Fuks, F. (2002) Dnmt3L is a transcriptional repressor that recruits histone deacetylase. Nucleic Acid Res. 30, n°17, 3831-3838.

• Thomas, T.O., Kjems, J. (2001) SF2/ASF binds to a splicing enhancer in the third HIV-1 tat exon and stimulates U2AF binding independently of the RS domain. J.Mol.Biol. 312, 649-662.

• Tange, T.O., Damgaard, C.K., Guth, S., Valcarel, J., Kjems, J. (2001) The HnRNP A1 protein regulates HIV-1 tat splicing via a novel intron silencer element. EMBO Journal 20, 5748-5758.

• Kyriakopoulou, C., Larsson, P., Liu, L., Schuster, J., Söderbom, F., Kvisebom, L., Virtanen, A. (2005) U1-like snRNAs lacking complementarity to canonical 5'splice sites RNA (submitted for publication).

• Brenner, C., Deplus, R., Didelot, C., Loriot, A., Viré, E., De Smet, C., Gutierrez, A., Danovi, D., Bernard, D., Boon, T., Pelicci, P.G., Amati, B., Kouzarides, T., de Launoit, Y., Di Croce, L. and Fuks, F. (2005) Myc represses transcription through recruitment of DNA methyltransferase corepressor. EMBO Journal 24, 336-346.

• Henry, C., Meda, P., Dasnois, L., Lebtahi, K., Malaisse, W.J., Remacle, C. and Miller, A.O.A. (2004) Culture of porcine neonate pancreatic cells onto two-dimensional microsupport; diabetes cell therapy with reconstituted organoids. Diabetes Research 38, 33-45.

• Marchand, V., Mougin, A., Mereau, A. and Branlant, C. (2005) Study of RNA-Protein interactions and RNA structure in RNP particules In: Westhof, Bindereif, Schön, Hartmann, (eds) Handbook of RNA Biochemistry, Chapter 11, 172-204, Wiley-VCH

• Hallay, H., Locker, N., Ayadi, L., Ropers, D., Guittet, E. and Branlant, C. (2006) Biochemical and NMR study on the competition between proteins SC35, SRp40 and hnRNP A1 at the HIV-1 Tat exon 2 splicing site. J. Biol. Chem., Vol. 281, Issue 48, 37159-37174

• Burgers, W.A., Blanchon, L., Pradhan, S., de Launoit, Y., Kouzarides, T., Fuks, F. (2006) Viral oncoproteins target the DNA methyltransferases. Oncogene

• Bernard, D., Gil, J., Dumont, P., Rizzo, S., Monté, D., Quatannens, B., Hudson, D., Visakorpi, T., Fuks, F. and de Launoit, Y.(2006) The methyl-CpG-binding protein MECP2 is required for prostate cancer cell growth. Oncogene 25, 1358–1366.

• Viré, E., Brenner, C., Deplus, R., Blanchon, L., Fraga, M., Didelot, C., Morey, L., Van Eynde, A., Bernard, D., Vanderwinden, J-M., Bollen, M., Esteller, M., Di Croce, L., de Launoit, Y., and Fuks, F. (2006) The Polycomb group protein EZH2 directly controls DNA methylation. Nature 439, 871-874.

• Hanna, C., Gjerde, D., Nguyen, L., Dickman, M., Brown, P. and Hornby, D. (2006) Micro-scale open-tube capillary separations of functional proteins. Analytical Biochemistry 350, 128-137.

• Jacquenet, S., Méreau, A., Bilodeau, P.S., Damier, L., Martin Stoltzfus, C. and Branlant, C. (2001) A Second Exon Splicing Silencer within Human Immunodeficiency Virus Type 1 tat Exon 2 Represses Splicing of Tat mRNA and Binds Protein hnRNP H*. J. Biol. Chem., 276, No 44, Issue of November 2, 40464-40475

• Azubel, M., Wolf, S.G., Sperling, J. and Sperling, R. (2004) Three-dimensional structure of the nature spliceosome by cryo-electron microscopy. Molec. Cell. 15, 833-839

• Hoffmann,M.H., Tuncel,J., Shriner,K., Tohidast-Akrad, M.Türk;B., Pinol-Roma,S., Serre, G., Schett,G., Smolen,J ;S., Holmdahl,R. and Steiner,G. (2007) The Rheumatoid Arthritis- Associated Autoantigen hnRNP-A2 (RA33) is a Major Stimulation of Autoimmunity in Rats with Pristane-induced Arthritis. The Journal of Immunology 179, 7568-7576

• Roche,K.C., Wiechens,N., Owen-Hughes,T and Perkins, N.D. (2004) The FHA domain protein SNIP1 is a regulator of the cell cycle and cyclin D1 expression. Oncogene 23, 8185-8195

• Rix,V., Hantschel,O., Dürnberger, G.,Lily,L.,Rix,R., Planyavsky,M., Fernbach,N.V., Kaupe,I.,Bennett,K.L., Valent,P., Colinge,J., Köcher,T. and Superti-Furga,G. (2007) Chemica proteomic profiles of the BCR-ABL inhibitors imatinib, nilotinib, and desatinib reveal novel kinase and non kinase targets Blood 110,4055-4063

• Sergeant,D., Moser, M., and Carvell, J. (2007) Measurement and Control of viable cell density in a mammalian cell bioprocessing facility: Validation of the software. Poster presented at the 20th ESACT meeting Dresden, 17-20th June 2007

• Sergeant, D., Vereecke, J., Moser, M., Carvell, J.P., Miller, A.O.A., Poppleton, J. Controlling a constant level of viable biomass in a perfused cytostat using radio-frequency impedance. Poster presented at Cell Engineering Conference, April ,2006.

• Brandt, D., Marion, S., Griffiths, G., Watanabe, T., Kaibuchi, K. and Grosse, R. (2007) Dia 1 and IQGAP1 interact in cell migration and phagocytic cup formation. Journal of Cell Biology 178, 193-200

• Ropers, D., Ayadi, L., Gattoni, R., Jacquenet, S., Damier, L., Branlant, C. and Stévenin, J. (2004) Differential effects of the SR proteins 9G8, SC35, ASF/SF2 and SRp40 on the utilization of the A1 to A5 splicing sites of HIV-1 RNA. J. Biol. Chem., 279, 29963-29973

• Gehring, N.H., Lamprinaki, S., Kulozik, A.E. and Hentze, M. (2009) Dissassembly of exon junction complexes by PYM. Cell 137, 536-548

• Gehring, N.H., Lamprinaki, S., Hentze, M. and Kulozik, A.E. (2009) The hierarchy of exon-junction complex assembly by the spliceosome explains key features of mammalian nonsense-mediated mRNA decay. Plos Biology 7 (5) e 1000120

• Agranat, L., Sperling, J. and Sperling, R. (2010) A novel tissue-specific alternatively spliced form of the A-to-I RNA editing enzyme ADAR2. RNA Biology 7, 1-10

• Rivera-Calzada, A., Spagnolo, L., Laurence H., P., Llorca, O. (2007) Structural model of full-lengh human Ku70-Ku80 heterodimer and its recognition of DNA and DNA-PKcs. EMBO reports 8, 56-62

• Stefan Reber, Jolanda Stettler1, Giuseppe Filosa, Martino Colombo Daniel Jutzi, Silvia C Lenzken, Christoph Schweingruber, Rémy Bruggmann, Angela Bachi, Silvia ML Barabino, Oliver Mühlemann & Marc-David Ruepp (2016) Minor intron splicing is regulated by FUS and affected by ALS-associated FUS mutants. The EMBO Journal 35: 1504–1521


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