HIGH QUALITY AT THE LOWEST COST

IpraCell has developed and reduced to practice a range of innovative and enabling technologies. This way, IpraCell is able to offer you high quality biologicals at the lowest cost.

REPRODUCIBLE RESULTS

Thanks to the IpraCell's innovative development and manufacturing process, all your results are reproducible between batches.

REFERENCES ALL AROUND THE WORLD

Our products are used worldwide by research or production entities for the study of biochemical processing, high throughput screening or purification of biological material from human origin.

K-562

The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises.
The cell population has been characterized as highly undifferentiated and of the granulocytic series.
Studies conducted by Anderson, et al., on the surface membrane properties led to the conclusion that the K-562 was a human erythroleukemia line. The K-562 cell line has attained widespread use as a highly sensitive in vitro target for the natural killer assay.
See Pross, et al. for a detailed analysis of the in vitro assay of NK cells including the mathematics of quantitation of NK cell activity.
K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic and monocytic series.
The effect of inducers on sublines derived from the original K-562 cell line have been reviewed by Koeffler and Golde. Cultures from the ATCC stock have been shown to exhibit this sensitivity for assessing human natural killer activity.
Karyological studies on various K-562 sublines have been classified into three groups (A,B,C) by Dimery, et al.
The strain obtained by the ATCC most closely resembles the B population. Occurrence of the Philadelphia chromosome, however, was of much lower frequency; none detected in 15 metaphases examined.

 

Cell Line
K-562
Product
Whole Cell Pellets
Quantity
5 x 10e9 cells
Reference
CC-08-10-50

 

Whole Cell Pellets:

 

Cells are harvested in exponential phase. Cell pellets are prepared by low speed centrifugation, rinsed with phosphate buffer saline, snap frozen in liquid nitrogen and stored at -120 degree Celsius. The cell pellets are not prepared in aseptic conditions and are not intended to be used as seed for a new culture.

 

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